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Sleep Rhythm Test 2 Dual Hormone Profile


Testing Cortisol + Melatonin (12-2am) Sleep hormone testing.

About the Sleep Rhythm Test V2 – Dual Hormone Profile:

Sample Collection: 1 simple saliva sample collection between 12PM and 2AM. Instructions included.

Results: Results in both chart and graph form. “Safe” and “Target” reference ranges apply for both cortisol and melatonin tests. Summary and indication included.

Short Overview: When functioning correctly, our pineal glands release melatonin into the bloodstream at night time causing drowsiness. However there is another hormone, cortisol, that has the ability to prevent melatonin from providing that assistance we need for sleep. As an antagonist, cortisol is too powerful for melatonin to ‘over-ride’. So if it’s active at night, that is if it’s observed in more than tiny amounts, we have a problem.

Why use this test? One of the more common reasons for using this test is investigation of long term, or chronic sleep disruption. Results from the Sleep Rhythm V2 – Dual Hormone Profile Test results will reveal intrusive and unwanted late night cortisol activity whilst also checking to see any effect on melatonin production.

Abnormal results may trigger further investigation and possible treatment. Naturally correcting, (i.e. non prescription treatment for) moderate hormone irregularity is possible through diet, exercise, sleep pattern training which includes evening sleep preparation (removing devices known to hinder sleep), and regular early rising times to coincide with sunrise.

Fasting Required: Yes – 1 hour. [Revised July 2019]

Instructions provided with all test kits.

Alternate test: Melatonin sleep rhythm test kit (includes X 2 melatonin @ midnight and between 6 – 8AM

This test is performed by NutriPATH®.


  1. Purchase test, have it delivered to your doorstep. Tracking number available on request. Now sent via Express Post 7 days a week.
  2. Perform simple saliva sample collection and complete the included referral form.
  3. Post your sample and referral form back to the lab’ using the Express Post satchel. Tracking included.
  4. Receive your hormone test results by secure .PDF to your designated email address.


I Am Taking Vitamins/Minerals. Would this Affect The Test Results?

Sustained high vitamin B12 supplementation is known to interfere with melatonin and it is advised that use of B12 is ceased 1 week prior to testing only if a 'clear result' is required. More information can bee found within your test kit package.

Taking Oral Contraceptive

We are not testing reproductive hormones so taking hormonal birth control fine.

I Have An Implanted IUD, Will I Be Able To Use The TestoChecker Kit?

Yes, however, if you plan on removing the IUD, at least 1 month should pass before using the TestoChecker Kit.

Do any medicines or supplements affect the results?

Generally speaking, hormone testing is fine to do when using medicines but there are exceptions. Any supplements used to mimic the effects of hormones, and any steroid based treatment will affect the results. Information contained within your kit outlines how to 'obtain a clear result' if this is required.

Please note: We do not recommend ceasing use of any prescribed medicines without first consulting your GP.

What are the most common causes of melatonin irregularity?

Melatonin levels can be affected by as specified caffeine, alcohol, tobacco but also high vitamin B12 supplementation, stress (increased cortisol levels), irregular sleeping patterns e.g. shift work, increased exposure to light – particularly Blue Light and excessive exercise can also deplete melatonin levels.

How long does testing take?
Generally speaking testing time is up to 10 working days. For more detailed information regarding testing time see here.


More questions and answers related to hormone testing in Australia.


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