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Peer Studies

Peer-Reviewed Studies of Salivary Sex Hormone Testing

2012 – Salivary hormone measurement using LC/MS/MS: specific and patient-friendly tool for assessment of endocrine function.

Saliva has recently been attracting attention as a patient-friendly available bio-fluid and an alternative to serum/plasma for hormone tests. LC coupled with atmospheric pressure ionization-MS/MS, especially electrospray ionization (ESI)-MS/MS, has been recently valued as a highly specific method in the analysis of salivary hormones. In this article, LC/ESI-MS/MS assays for salivary hormones are overviewed according to the papers that have been published during the last 5 years. Practical derivatization to enhance the detectabilities of hormones in ESI-MS/MS is also discussed, because a major disadvantage of using saliva is low hormone concentrations.

Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda, Japan. PMID: 22975487

2009 – Salivary sex hormone measurement in a national, population-based study of older adults

This study was “the first large, population-based study of older adults to measure salivary estradiol, progesterone, dehydroepiandrosterone (DHEA), and, in women, testosterone.”

The researchers analysed self-collected saliva specimens from 2,772 U.S. men and women, from 57-85 years old.

Conclusion: “These data demonstrate a high cooperation rate with in-home salivary specimen collection from older adults and good validity of sex hormone measurements.”

J Gerontol B Psychol Sci Soc Sci. 2009 Nov;64 Suppl 1:i94-105. Epub 2009 Feb 9.Salivary sex hormone measurement in a national, population-based study of older adults. Gavrilova N, Lindau ST. Department of Obstetrics and Gynecology, University of Chicago, 5841 S. Maryland Avenue, MC2050, Chicago, IL 60637, USA. PMID 19204073

2009 – Simultaneous determination of salivary testosterone and dehydroepiandrosterone using LC-MS/MS

The researchers measured testosterone and DHEA levels in saliva using the liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-ESI-MS/MS) method to determine if hormone levels were sufficiently accurate to screen for late-onset hypogonadism (LOH), i.e., insufficient testosterone.

Conclusion: Saliva testing was found to be 98.5-101.8% accurate in measuring testosterone and DHEA levels, and “thus, the developed method has satisfactory applicability in the diagnosis and medication for LOH.”

J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Sep 1;877(25):2615-23. Epub 2008 Nov 5. Simultaneous determination of salivary testosterone and dehydroepiandrosterone using LC-MS/MS: Method development and evaluation of applicability for diagnosis and medication for late-onset hypogonadism. Shibayama Y, Higashi T, Shimada K, Odani A, Mizokami A, Konaka H, Koh E, Namiki M. Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan. PMID 19010090

2007 – Salivary testosterone: a reliable approach to the diagnosis of male hypogonadism

The researchers studied whether measurements of salivary testosterone (Sal-T) matched measurements of serum (blood) free testosterone. They compared the Sal-T and serum testosterone levels in 20 men with hypogonadism (insufficient testosterone) and 52 men with normal testosterone levels.

Conclusion: “The results support the inclusion of this biomarker [salivary testosterone] as a noninvasive approach in the diagnosis of male androgen deficiency.”

Clin Endocrinol (Oxf). 2007 Nov;67(5):656-62. Salivary testosterone: a reliable approach to the diagnosis of male hypogonadism. Arregger AL, Contreras LN, Tumilasci OR, Aquilano DR, Cardoso EM. Endocrine Research Department, Instituto de Investigaciones Médicas A. Lanari, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina. PMID 17953627

2006 – Validation of salivary testosterone as a screening test for male hypogonadism

The researchers performed two studies of saliva testing to screen for male hypogonadism, i.e., insufficient testosterone levels.

The first study of 1,454 men found expected age-related testosterone variance by age.

The second study found that “salivary testosterone was strongly correlated with bioavailable testosterone, … calculated free testosterone, … and total testosterone.”

Conclusion: “These studies support the use of salivary testosterone as an acceptable assay for screening for hypogonadism.”

Aging Male. 2006 Sep;9(3):165-9. Validation of salivary testosterone as a screening test for male hypogonadism. Morley JE, Perry HM 3rd, Patrick P, Dollbaum CM, Kells JM. Division of Geriatric Medicine, Saint Louis University, Missouri 63104, USA. PMID 17050116

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