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Saliva Testing For Hormone Levels

At home hormone saliva testing is widely accepted in the medical community as a reliable and accurate method to determine hormonal profile, providing a comprehensive snapshot of hormone production from multiple endocrine organs.


The endocrine organs, such as the sex organs (ovaries and testes), the adrenal glands, and the pineal gland (located in the brain), synthesise and secrete hormones into the blood stream which travel around the body to carry out various physiological function

There are several benefits of using at home hormone saliva tests to identify hormonal imbalance. It is convenient, results are obtained quickly and self-initiated testing enables one to take control of their hormone balance and health.

The most palpable benefit of at-home hormone balance testing is the convenience. It requires minimal time, is non-invasive, doesn’t require making appointments with a GP and is affordable. The Testing Kit, including the Referral Form, is express posted same day to the designated address. Following the instruction provided, a series of saliva samples are collected. These samples and the referral form are returned to the laboratory using the prepaid express satchel provided. The desired hormones are measured in a laboratory and the results sent to you by TestoChecker Hormone Test Tits the moment that are available. A summary of each individual hormone reported  is included with your results.

First-line test

Our hormones control many aspects of our health and, quite simply, how good we feel. Today, it has never been easier to take control of our healthy wellbeing and to be empowered not only through making the right lifestyle choices but taking steps to investigate why we don’t feel well and what can be done about it.

Persistent symptoms of hormone imbalance or deficiency may relate to feelings of fatigue, low mood, anxiety, loss of sex drive, irregular periods, and difficulty losing weight – the list goes on.

DIY at-home hormone saliva testing is an easy and reliable way to obtain a first line indication of any underlying hormonal imbalance.


Salivary hormone balance tests provide a convenient way of keeping track of hormone levels when baseline or previous testing has already been done, or pre-existing conditions warrant regular measurements of hormone balance.

Monitoring of hormone balance could be required following interventions such as hormone supplementation or hormone replacement therapy, or to assess ovarian function or menstrual irregularities, or even to evaluate hormonal profiles following lifestyle changes.

Testing in such cases is a useful way to make sure hormonal balance is within the healthy or expected range.

Many of the hormones that regulate our physiology, including reproductive function or the ability to control stress are not steady-state, but fluctuate in a daily or monthly rhythm. At home hormone saliva testing permits a detailed assessment of true hormone levels at multiple points in time.

Multi-Point Saliva Collection Method [MSM], provides a broader measure, allowing for a wider view and more thorough observation of the hormone secretion pattern. This applies to both the androgen and stress hormones. Cortisol testing is available in various sample collection time configuration depending on need, and is a great example of the benefit of multi-point saliva testing for checking hormone balance.

The hormones cortisol and melatonin are pulsatile and secreted in a daily ‘circadian’ rhythm. Testing them involves saliva collections at six hour intervals [usually]. It is the pattern of secretion that paints the full picture of their performance. With the androgens, i.e. testosterone, progesterone, the estrogens and precursor hormone DHEAs, it is the morning behaviour which is of interest. This is when these hormones should be most active. Saliva samples can be collected over a two hour period (Cortisol awakening response test) or over a day (Comprehensive cortisol test), allowing for the laboratory to map hormone secretion patterns to better asses hormone health.

Multiple saliva collections also reduces the possibility of “random” contamination during collection affecting the test result. Saliva samples which appear compromised are set aside, and testing of the remaining samples continues.

Steroid hormones including, but not limited to, androstenedione, testosterone, estrogens, progesterone and cortisol, are for the most part locked down by binding proteins in the blood. In this state, the hormones are biologically inactive: the binding proteins prevent hormone overactivity by preventing the hormone attaching to its receptor.

Only a small fraction of a hormone (~5%) is released by the binding protein for action, and it is this hormone fraction that is detected in saliva. Hormones secreted into the saliva are ‘free’, and give an accurate representation of biologically active hormone balance (levels) at the time of the test.

The latest analytical technologies in hormone-detection assays are used in the laboratory to yield highly precise and sensitive test results. This means that very low levels of hormones can be detected with an accurate read-out of hormone concentration. As such one saliva sample can be portioned across multiple hormone tests allowing for combined testing and providing a more comprehensive hormone profile that is provided in a single report.

The rigorous clinical diagnostic practices applied to salivary hormone testing provides accurate medical information that can be passed to your GP for more thorough investigations directed by your medical professional.

At TestoChecker your saliva samples are in safe hands. TestoChecker will only partner with NATA-accredited laboratories.

NATA (National Association of Testing Authorities) is an independent authority that assess and award accreditation status to laboratory facilities. NATA experts inspect multiple aspects of laboratory operations to ensure that data are high-quality, accurate and collected following Good Laboratory Practice guidelines.

NATA accreditation, which is endorsed by the Australian government, ensures that the laboratory is compliant with industry standards across Australia. At TestoChecker, we are committed to providing our customers with high-quality, reliable tests to the highest possible standards.

At TestoChecker there is full flexibility to design the hormone balance test that’s right for you. This allows you to uniquely tailor your tests to your needs. Using the Design Your Own Hormone Test tool, selection of multiple hormones can be chosen that are either measured once off to give a baseline value or can be measured in multiples over-time. In the cases of episodic hormones that fluctuate over the course of a day, up to 4 tests can be done at desired time-points, to return a detailed assessment of hormone patterns.

Common questions about Saliva testing

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Client Reviews

  • Great service and very happy I invested the time and money.

    Rosie Fletcher

  • Amazing customer service -Michelle went the extra mile to make sure I had all the information I required and understood everything. very prompt with responses. Highly recommend.

    Vanessa Donley

  • Found this very useful. Easier than I thought it would be!

    Jason A

  • It was easy and quick!

    Patrick Van Eybergen

  • Really amazing service !! Michelle was absolutely fantastic throughout the entire process, kept me updated all the time, responded quickly to my queries and followed up on my results. Thank you so much, I will be back!

    Sean M

  • My wife and I have both purchased a number of single and multiple combination hormone tests..and used them over a period of 6 months. The TestoChecker team consistently provided (at a very competitive price) awesome, professional and friendly customer service at or above what you would expect from a premium service.....

    Gav and Kylie

  • Testochecker provides a fantastic service! Getting your hormones checked is SO important. ...(cont')

    Mike S

  • A couple of very minor hiccups but overall the customer service is excellent. I will definitely be using them again.

    Maurice Burrows

  • TestoChecker's customer service was amazing! TestoChecker staff went the extra mile to ensure that I received my results as soon as it was available. Great work!

    Gurdip Hans

  • Super quick results from interstate and fantastic service.... Thanks so much to you and your team!

    Lisa Thatcher

  • Thoroughly recommend Testochecker. Staff have given over and above expectations. Would encourage anyone needing help of this nature to do so as they certainly won’t be disappointed. Great professional company.

    Margaret Cooper

  • Received my results quickly and all my questions were answered by the very helpful staff. Highly recommend using their service if you're considering it.

    Ray S

  • 5 Stars.