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Melatonin Sleep Rhythm Test Kit


Melatonin hormone test (X2) 12PM and 6AM

About this Hormone Test Kit:

Sample Collection: 2 sample collections at both 12PM and 6AM.

Results: Results in both chart and graph form.

Short Overview: When functioning correctly, our pineal glands release melatonin into the bloodstream at night time causing drowsiness. When not functioning correctly, sleep may be disrupted or sporadic. This can be a result of many possible factors including long term sleep disruption due to work, lifestyle or health. Melatonin also regulates pathways central to the immune response while abnormal melatonin secretion alters the immune function.

Why use this test? Results from the Melatonin Sleep Rhythm Test Kit will reveal if your Melatonin production is in sync with the day night cycle known as the ‘Circadian Rhythm’ or rather a Free Running State. Abnormal results may trigger further investigation and possible treatment.

Fasting Required: Yes – 1 hour. [Revised July 2019]

Instructions provided with all test kits.

This test is performed by NutriPATH®.

Alternate tests:

Sleep Rhythm Test 2 Dual Hormone Profile 

Stress and Sleep Hormone Test


Simple 3 Step Process:

  1.  Purchase test. Sent via Express Post same day Mon-Fri, <5PM AEST. Tracking number available on request.
  2.  Perform simple saliva sample collection as per instruction provided.
  3.  Post your sample and referral form back to the lab’ using the Express Post satchel. (Tracking included)

Receive your hormone test results by secure .PDF to your designated email address.


I Am Taking Vitamins/Minerals. Would this Affect The Test Results?

Sustained high vitamin B12 supplementation is known to interfere with melatonin and it is advised that use of B12 is ceased 1 week prior to testing only if a 'clear result' is required. More information can bee found within your test kit package.

Taking Oral Contraceptive

We are not testing reproductive hormones so taking hormonal birth control fine.

I Have An Implanted IUD, Will I Be Able To Use The TestoChecker Kit?

Yes, however, if you plan on removing the IUD, at least 1 month should pass before using the TestoChecker Kit.

Do any medicines or supplements affect the results?

Generally speaking, hormone testing is fine to do when using medicines but there are exceptions. Any supplements used to mimic the effects of hormones, and any steroid based treatment will affect the results. Information contained within your kit outlines how to 'obtain a clear result' if this is required.

Please note: We do not recommend ceasing use of any prescribed medicines without first consulting your GP.

What are the most common causes of melatonin irregularity?

Melatonin levels can be affected by as specified caffeine, alcohol, tobacco but also high vitamin B12 supplementation, stress (increased cortisol levels), irregular sleeping patterns e.g. shift work, increased exposure to light – particularly Blue Light and excessive exercise can also deplete melatonin levels.

How long does testing take?
Generally speaking testing time is up to 10 working days. For more detailed information regarding testing time see here.


More questions and answers related to hormone testing in Australia.