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Stress and Sleep Hormone Test


Cortisol X 2 AM/PM, Melatonin X 1 PM. (Includes Ratios)

About this test


Hormones tested: Cortisol X 2 AM/PM, Melatonin X 1 PM.


Sample collection: X 2 Saliva. Detailed instructions included.


Results: The results for this test are displayed in graph and chart form. Cortisol / melatonin ratios provided.


N.B. Saliva testing (Salivaomics), has evolved over time and is now the chosen test biological specimen type for cortisol and melatonin levels. [1]

Why use this test?


This test measures the behaviour of two hormones responsible for a range of functions. Aside from the numerous physical implications of cortisol and melatonin, they ultimately impact upon our mood, good or bad. This alone means both hormones are vital for healthy mental and physical function in the modern world. As much as the old, where our cortisol played a vital role in keeping us alert and safe.

Stress and Sleep Hormone Test Includes:

Cortisol test


This component of the test is used to identify abnormal cortisol output and provides assessment via 2 ‘snapshots’, of the cortisol daily pattern which are displayed in graph form.


Why test cortisol? Cortisol is great when it is working for us but not so great when it isn’t. Healthy cortisol assists with our busy daily lifestyle and also when confronting stressful situations, which we all face from time to time. Issues arise when it malfunctions, which can be over or under production, or where levels are elevated at the time we are attempting to sleep.


Melatonin test


Why test Melatonin? Test results will reveal if your Melatonin production is taking place as it should be at night. Abnormal results, depending on severity, may trigger further investigation and possible treatment. Slightly low or high melatonin levels can often be corrected by following simple steps.



  1. Purchase test, have it delivered to your doorstep. Tracking number available on request. Now sent via Overnight Express.
  2. Perform simple saliva sample collection and complete your referral form.
  3. Post your sample and referral form back to the lab’ using the Overnight Express satchel. Tracking included.
  4. Receive your hormone test results by secure .PDF to your designated email address.


More on Melatonin: When functioning correctly, our pineal glands release melatonin into the bloodstream at night time causing drowsiness. When not functioning correctly, sleep may be disrupted or sporadic. This can be a result of many possible factors including long term sleep disruption due to work, lifestyle, health and even use of vitamin B12. Melatonin also regulates pathways central to the immune response [cell repair between ‘shifts’] while abnormal melatonin secretion alters the immune function.


[1]Source: “Cortisol and melatonin aberrations”. – Wikipedia, | Source: “Salivaomics and saliva testing”. – Wikipedia,

I Am Taking Vitamins/Minerals. Would this Affect The Test Results?

Sustained high vitamin B12 supplementation is known to interfere with melatonin and it is advised that use of B12 is ceased 1 week prior to testing only if a 'clear result' is required. More information can bee found within your test kit package.

Taking Oral Contraceptive

We are not testing reproductive hormones so taking hormonal birth control fine.

I Have An Implanted IUD, Will I Be Able To Use The TestoChecker Kit?

Yes, however, if you plan on removing the IUD, at least 1 month should pass before using the TestoChecker Kit.

Do any medicines or supplements affect the results?

Generally speaking, hormone testing is fine to do when using medicines but there are exceptions. Any supplements used to mimic the effects of hormones, and any steroid based treatment will affect the results. Information contained within your kit outlines how to 'obtain a clear result' if this is required.

Please note: We do not recommend ceasing use of any prescribed medicines without first consulting your GP.

What are the most common causes of melatonin irregularity?

Melatonin levels can be affected by as specified caffeine, alcohol, tobacco but also high vitamin B12 supplementation, stress (increased cortisol levels), irregular sleeping patterns e.g. shift work, increased exposure to light – particularly Blue Light and excessive exercise can also deplete melatonin levels.

How long does testing take?
Generally speaking testing time is up to 10 working days. For more detailed information regarding testing time see here.
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