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Saliva Hormone Testing

A closer look at Saliva Hormone Testing

There is now sufficient evidence to show that standard serum (blood) testing protocols cannot quantify low concentrations of hormones in a reliable way. In general, blood measurements only give the total saliva hormone concentration, whereas saliva testing measures the important free, bioavailable, hormone level. 

The measurement of testosterone (T) in plasma or serum, as done in most laboratories, suffers from a number of serious problems.” – Endocrine Society Position Statement [Scroll down to link]


At present most saliva hormone testing is conducted using the traditional method, i.e., by sampling and analysing blood – an invasive and costly procedure. Tandem.Mass Spectrometer.Research has shown that, for most purposes involving otherwise healthy individuals, this method has limited application. Here’s why:

Saliva hormones present in the blood are either completely “bound” to their specific binding globulins, or “free”.

The bound component is biologically inactive and is considered to be a kind of hormone storage. Only less than 5% of the total hormones present are “free”, “active” or “bioavailable” and these are responsible for hormone activity in the body.

In serum (blood) or plasma testing, the saliva measurement represents the level of the total hormone, including the “bound” component. This total concentration at best gives only an approximate indication of the bioavailable hormone present in cases of completely healthy and normal patients.

The Endocrine Society published an official position statement*, including that reliable measurements in serum testing either need an additional extraction step, or need to be completed using chromatographic methods, such as tandem MS or GCMS (super sophisticated and expensive equipment). 

This casts doubt on the validity of blood testing for free, bioavailable hormones.

On the other hand, saliva sampling is non-invasive and can be done anytime, anywhere at multiple time points day or overnight. Provided the proper collection and testing procedure(s) is observed, hormones tested reliably include:

More about using saliva for Hormone testing?

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